› 2d3l

X-ray diffraction
2.3Å resolution

Crystal structure of maltohexaose-producing amylase from Bacillus sp.707 complexed with maltopentaose.

Released:
DOI: 10.2210/pdb2d3l/pdb
PDB entry 2d3l coloured by chain, front view.
PDB entry 2d3l coloured by chain, side view.
PDB entry 2d3l coloured by chain, top view.
Source organism: Bacillus sp. 707
Primary publication:
Role of Trp140 at subsite -6 on the maltohexaose production of maltohexaose-producing amylase from alkalophilic Bacillus sp.707.
Kanai R, Haga K, Akiba T, Yamane K, Harata K
Protein Sci 15 468-77 (2006)
PMID: 16452622

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-alpha-D-glucosidic linkages in amylaceous polysaccharides, to remove successive maltohexaose residues from the non-reducing chain ends
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148647 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Glucan 1,4-alpha-maltohexaosidase Chain: A
Molecule details ›
Chain: A
Length: 485 amino acids
Theoretical weight: 55.44 KDa
Source organism: Bacillus sp. 707
Expression system: Bacillus subtilis
UniProt:
  • Canonical: P19571 (Residues: 34-518; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
3 bound ligands:
Ligand GLC 2 x GLC
Ligand CA 3 x CA
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-18B
Spacegroup: P212121
Unit cell:
a: 47.45Å b: 82.46Å c: 126.91Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 0.168 0.213
Expression system: Bacillus subtilis

Quick links

Citations

1 review citation

Occurrence and functional significance of secondary carbohydrate binding sites in glycoside hydrolases.
Cuyvers et al. (2012)
1 other article

1 mention without citation

Role of Trp140 at subsite -6 on the maltohexaose production of maltohexaose-producing amylase from alkalophilic Bacillus sp.707.
Kanai et al. (2006)