PDBe 2d0n

X-ray diffraction
1.57Å resolution

Crystal structure of the C-terminal SH3 domain of the adaptor protein GADS in complex with SLP-76 motif peptide reveals a unique SH3-SH3 interaction


Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
SLP-76 binding peptide Chains: B, D
Molecule details ›
Chains: B, D
Length: 9 amino acids
Theoretical weight: 1 KDa
Source organism: Synthetic construct
Expression system: Not provided
GRB2-related adaptor protein 2 Chains: A, C
Molecule details ›
Chains: A, C
Length: 59 amino acids
Theoretical weight: 6.72 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
  • Canonical: O89100 (Residues: 267-322; Coverage: 17%)
Gene names: Gads, Grap2, Grb2l, Grid, Mona
Sequence domains: SH3 domain
Structure domains: SH3 Domains

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P42212
Unit cell:
a: 58.622Å b: 58.622Å c: 74.098Å
α: 90° β: 90° γ: 90°
R R work R free
0.221 0.221 0.284
Expression systems:
  • Not provided
  • Escherichia coli BL21(DE3)