› 2cxi

X-ray diffraction
1.94Å resolution

Crystal Structure Of An N-terminal Fragment Of The Phenylalanyl-tRNA Synthetase Beta-Subunit From Pyrococcus Horikoshii

Released:
DOI: 10.2210/pdb2cxi/pdb
PDB entry 2cxi coloured by chain, front view.
PDB entry 2cxi coloured by chain, side view.
PDB entry 2cxi coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Primary publication:
Structural and mutational studies of the amino acid-editing domain from archaeal/eukaryal phenylalanyl-tRNA synthetase.
Sasaki HM, Sekine S, Sengoku T, Fukunaga R, Hattori M, Utsunomiya Y, Kuroishi C, Kuramitsu S, Shirouzu M, Yokoyama S
Proc Natl Acad Sci U S A 103 14744-9 (2006)
PMID: 17003130

Function and Biology Details

Reaction catalysed: 
ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130748 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phenylalanine--tRNA ligase beta subunit Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 348 amino acids
Theoretical weight: 40.92 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O73984 (Residues: 1-348; Coverage: 63%)
Gene names: PH0657, pheT
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 27 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: C2
Unit cell:
a: 142.779Å b: 81.973Å c: 139.096Å
α: 90° β: 116.79° γ: 90°
R-values:
R R work R free
0.19 0.188 0.231
Expression system: Escherichia coli

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Citations

3 review citations

Aminoacyl-tRNA synthesis and translational quality control.
Ling et al. (2009)
2 more

5 mentions without citation

Occludin controls HIV transcription in brain pericytes via regulation of SIRT-1 activation.
Castro et al. (2016)
4 more