PDBe 2col

X-ray diffraction
2.2Å resolution

Crystal structure analysis of CyaA/C-Cam with Pyrophosphate

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calmodulin-2 A Chain: B
Molecule details ›
Chain: B
Length: 67 amino acids
Theoretical weight: 7.74 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DP34 (Residues: 80-146; Coverage: 45%)
Gene name: calm2-a
Structure domains: EF-hand
Calmodulin-sensitive adenylate cyclase Chain: A
Molecule details ›
Chain: A
Length: 356 amino acids
Theoretical weight: 38.56 KDa
Source organism: Bordetella pertussis
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DKX7 (Residues: 7-362; Coverage: 21%)
Gene names: BP0760, cya, cyaA
Sequence domains: Anthrax toxin LF subunit
Structure domains: Adenylylcyclase toxin superfamily

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P41212
Unit cell:
a: 79.613Å b: 79.613Å c: 139.465Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.28 0.24 0.293
Expression system: Escherichia coli