› 2cmn

X-ray diffraction
2.3Å resolution

A Proximal Arginine Residue in the Switching Mechanism of the FixL Oxygen Sensor

Released:
DOI: 10.2210/pdb2cmn/pdb
PDB entry 2cmn coloured by chain, front view.
PDB entry 2cmn coloured by chain, side view.
PDB entry 2cmn coloured by chain, top view.
Source organism: Bradyrhizobium japonicum
Primary publication:
A proximal arginine R206 participates in switching of the Bradyrhizobium japonicum FixL oxygen sensor.
Gilles-Gonzalez MA, Caceres AI, Sousa EH, Tomchick DR, Brautigam C, Gonzalez C, Machius M
J Mol Biol 360 80-9 (2006)
PMID: 16813836

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149897 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sensor protein FixL Chain: A
Molecule details ›
Chain: A
Length: 130 amino acids
Theoretical weight: 14.7 KDa
Source organism: Bradyrhizobium japonicum
Expression system: Escherichia coli
UniProt:
  • Canonical: P23222 (Residues: 141-270; Coverage: 26%)
Gene names: bll2760, fixL
Sequence domains: PAS fold
Structure domains: PAS domain

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: R32
Unit cell:
a: 127.117Å b: 127.117Å c: 58.569Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.197 0.195 0.22
Expression system: Escherichia coli

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Citations

3 review citations

Bacterial sensor kinases: diversity in the recognition of environmental signals.
Krell et al. (2010)
2 more