› 2cmg

X-ray diffraction
2Å resolution

Crystal Structure of Spermidine Synthase from Helicobacter Pylori

Released:
DOI: 10.2210/pdb2cmg/pdb
PDB entry 2cmg coloured by chain, front view.
PDB entry 2cmg coloured by chain, side view.
PDB entry 2cmg coloured by chain, top view.
Source organism: Helicobacter pylori 26695
Primary publication:
Crystal structure of Helicobacter pylori spermidine synthase: a Rossmann-like fold with a distinct active site.
Lu PK, Tsai JY, Chien HY, Huang H, Chu CH, Sun YJ
Proteins 67 743-54 (2007)
PMID: 17357156

Function and Biology Details

Reaction catalysed: 
S-adenosyl 3-(methylthio)propylamine + putrescine = 5'-S-methyl-5'-thioadenosine + spermidine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127758 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyamine aminopropyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 262 amino acids
Theoretical weight: 30.59 KDa
Source organism: Helicobacter pylori 26695
Expression system: Escherichia coli
UniProt:
  • Canonical: O25503 (Residues: 1-262; Coverage: 100%)
Gene names: HP_0832, speE
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL17B2
Spacegroup: P21
Unit cell:
a: 61.147Å b: 58.141Å c: 74.832Å
α: 90° β: 90.93° γ: 90°
R-values:
R R work R free
0.216 0.216 0.273
Expression system: Escherichia coli

Quick links

Citations

3 review citations

A multifaceted role for polyamines in bacterial pathogens.
Shah et al. (2008)
2 more

3 mentions without citation

Ctf4 organizes sister replisomes and Pol α into a replication factory.
Yuan et al. (2019)
2 more