PDBe 2ckr

X-ray diffraction
1.77Å resolution

X-RAY CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF THERMOBIFIDA FUSCA ENDOGLUCANASE CEL5A (E5) E355Q IN COMPLEX WITH CELLOTETRAOSE

Released:
Source organism: Thermobifida fusca
Entry authors: Berglund GI, Gualfetti PJ, Requadt C, Gross LS, Bergfors T, Shaw A, Saldajeno M, Mitchinson C, Sandgren M

Function and Biology Details

Reaction catalysed:
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans. 
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase E-5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 306 amino acids
Theoretical weight: 34.18 KDa
Source organism: Thermobifida fusca
Expression system: Bacillus subtilis
UniProt:
  • Canonical: Q01786 (Residues: 161-466; Coverage: 71%)
Gene name: celE
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 49.365Å b: 70.85Å c: 76.278Å
α: 90° β: 93.86° γ: 90°
R-values:
R R work R free
0.16 0.158 0.193
Expression system: Bacillus subtilis