PDBe 2cjw

X-ray diffraction
2.1Å resolution

Crystal structure of the small GTPase Gem (GemDNDCaM) in complex to Mg.GDP

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GTP-binding protein GEM Chain: A
Molecule details ›
Chain: A
Length: 192 amino acids
Theoretical weight: 22.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P55040 (Residues: 70-261; Coverage: 65%)
Gene names: GEM, KIR
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
GTP-binding protein GEM Chain: B
Molecule details ›
Chain: B
Length: 192 amino acids
Theoretical weight: 22.43 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P55040 (Residues: 70-261; Coverage: 65%)
Gene names: GEM, KIR
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P61
Unit cell:
a: 116.617Å b: 116.617Å c: 81.409Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.209 0.241
Expression system: Escherichia coli