PDBe 2cit

X-ray diffraction
1.4Å resolution

Structure of the covalent intermediate of a family 26 lichenase

Released:

Function and Biology Details

Reaction catalysed:
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans. 
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase H Chain: A
Molecule details ›
Chain: A
Length: 282 amino acids
Theoretical weight: 32.06 KDa
Source organism: Ruminiclostridium thermocellum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P16218 (Residues: 25-304; Coverage: 30%)
Gene names: Cthe_1472, celH
Sequence domains: Glycosyl hydrolase family 26
Structure domains: Glycosidases

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 49.047Å b: 62.83Å c: 78.057Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.151 0.179
Expression system: Escherichia coli BL21(DE3)