› 2ch7

X-ray diffraction
2.5Å resolution

Crystal structure of the cytoplasmic domain of a bacterial chemoreceptor from Thermotoga maritima

Released:
DOI: 10.2210/pdb2ch7/pdb
PDB entry 2ch7 coloured by chain, front view.
PDB entry 2ch7 coloured by chain, side view.
PDB entry 2ch7 coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Reconstruction of the chemotaxis receptor-kinase assembly.
Park SY, Borbat PP, Gonzalez-Bonet G, Bhatnagar J, Pollard AM, Freed JH, Bilwes AM, Crane BR
Nat Struct Mol Biol 13 400-7 (2006)
PMID: 16622408

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194728 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Methyl-accepting chemotaxis protein 2 Chain: A
Molecule details ›
Chain: A
Length: 309 amino acids
Theoretical weight: 33.4 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9X0M7 (Residues: 222-529; Coverage: 58%)
Gene names: TM_1143, mcp2
Sequence domains: Methyl-accepting chemotaxis protein (MCP) signalling domain
Structure domains: Methyl-accepting chemotaxis protein
Methyl-accepting chemotaxis protein 2 Chain: B
Molecule details ›
Chain: B
Length: 309 amino acids
Theoretical weight: 33.41 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9X0M7 (Residues: 222-529; Coverage: 58%)
Gene names: TM_1143, mcp2
Sequence domains: Methyl-accepting chemotaxis protein (MCP) signalling domain
Structure domains: Methyl-accepting chemotaxis protein

Ligands and Environments

1 bound ligand:
Ligand PB 2 x PB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 24.6Å b: 99.4Å c: 117.2Å
α: 90° β: 90.5° γ: 90°
R-values:
R R work R free
0.259 0.259 0.297
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

21 review citations

Bacterial chemoreceptors: high-performance signaling in networked arrays.
Hazelbauer et al. (2008)
20 more

25 mentions without citation

Suprabasin-A Review.
Pribyl et al. (2021)
24 more