PDBe 2cci

X-ray diffraction
2.7Å resolution

Crystal structure of phospho-CDK2 Cyclin A in complex with a peptide containing both the substrate and recruitment sites of CDC6

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cyclin-A2 Chains: B, D
Molecule details ›
Chains: B, D
Length: 258 amino acids
Theoretical weight: 29.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P20248 (Residues: 175-432; Coverage: 60%)
Gene names: CCN1, CCNA, CCNA2
Sequence domains:
Structure domains: Cyclin A; domain 1
Cyclin-dependent kinase 2 Chains: A, C
Molecule details ›
Chains: A, C
Length: 299 amino acids
Theoretical weight: 34.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P24941 (Residues: 1-298; Coverage: 100%)
Gene names: CDK2, CDKN2
Sequence domains: Protein kinase domain
Structure domains:
Cell division control protein 6 homolog Chains: F, I
Molecule details ›
Chains: F, I
Length: 30 amino acids
Theoretical weight: 3.44 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q99741 (Residues: 71-100, 73-100; Coverage: 5%)
Gene names: CDC18L, CDC6

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 74.42Å b: 114.389Å c: 170.709Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.264 0.261 0.321
Expression systems:
  • Escherichia coli
  • Not provided