› 2cc1

X-ray diffraction
2.13Å resolution

Crystal structure of the class A beta-lactamase from Mycobacterium fortuitum

Released:
DOI: 10.2210/pdb2cc1/pdb
PDB entry 2cc1 coloured by chain, front view.
PDB entry 2cc1 coloured by chain, side view.
PDB entry 2cc1 coloured by chain, top view.
Source organism: Mycolicibacterium fortuitum
Primary publication:
Crystal structure of the Mycobacterium fortuitum class A beta-lactamase: structural basis for broad substrate specificity.
Sauvage E, Fonzé E, Quinting B, Galleni M, Frère JM, Charlier P
Antimicrob Agents Chemother 50 2516-21 (2006)
PMID: 16801434

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176803 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chain: A
Molecule details ›
Chain: A
Length: 262 amino acids
Theoretical weight: 28.11 KDa
Source organism: Mycolicibacterium fortuitum
Expression system: Mycolicibacterium smegmatis
UniProt:
  • Canonical: Q59517 (Residues: 33-294; Coverage: 100%)
Gene name: blaF
Sequence domains:
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LURE BEAMLINE DW32
Spacegroup: P212121
Unit cell:
a: 35.66Å b: 73.42Å c: 91.32Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.167 0.224
Expression system: Mycolicibacterium smegmatis

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Citations

4 review citations

Class A carbapenemases.
Walther-Rasmussen et al. (2007)
3 more

7 mentions without citation

Is it time for artificial intelligence to predict the function of natural products based on 2D-structure.
Liu et al. (2019)
6 more