PDBe 2cbu

X-ray diffraction
1.85Å resolution

Beta-glucosidase from Thermotoga maritima in complex with castanospermine

Released:

Function and Biology Details

Reaction catalysed:
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-glucosidase A Chains: A, B
Molecule details ›
Chains: A, B
Length: 468 amino acids
Theoretical weight: 53.94 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q08638 (Residues: 2-446; Coverage: 100%)
Gene name: bglA
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 94.076Å b: 94.521Å c: 113.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.183 0.237
Expression system: Escherichia coli BL21