› 2c9w

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF SOCS-2 IN COMPLEX WITH ELONGIN-B AND ELONGIN-C AT 1.9A RESOLUTION

Released:
DOI: 10.2210/pdb2c9w/pdb
PDB entry 2c9w coloured by chain, front view.
PDB entry 2c9w coloured by chain, side view.
PDB entry 2c9w coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the SOCS2-elongin C-elongin B complex defines a prototypical SOCS box ubiquitin ligase.
Bullock AN, Debreczeni JE, Edwards AM, Sundström M, Knapp S
Proc Natl Acad Sci U S A 103 7637-42 (2006)
PMID: 16675548

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-126990 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Suppressor of cytokine signaling 2 Chain: A
Molecule details ›
Chain: A
Length: 169 amino acids
Theoretical weight: 19.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O14508 (Residues: 30-198; Coverage: 85%)
Gene names: CIS2, SOCS2, SSI2, STATI2
Sequence domains:
Structure domains:
Elongin-B Chain: B
Molecule details ›
Chain: B
Length: 118 amino acids
Theoretical weight: 13.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15370 (Residues: 1-118; Coverage: 100%)
Gene names: ELOB, TCEB2
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Elongin-C Chain: C
Molecule details ›
Chain: C
Length: 97 amino acids
Theoretical weight: 10.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q15369 (Residues: 17-112; Coverage: 86%)
Gene names: ELOC, TCEB1
Sequence domains: Skp1 family, tetramerisation domain
Structure domains: Potassium Channel Kv1.1; Chain A

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand NI 3 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P3221
Unit cell:
a: 105.29Å b: 105.29Å c: 70.2Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.185 0.225
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

31 review citations

SOCS proteins, cytokine signalling and immune regulation.
Yoshimura et al. (2007)
30 more

20 mentions without citation

Inhibition of IL-6 family cytokines by SOCS3.
Babon et al. (2014)
19 more