Function and Biology

Structure of Trypanosoma brucei pteridine reductase (PTR1) in ternary complex with cofactor and the antifolate methotrexate

Source organism: Trypanosoma brucei brucei
Biochemical function: nucleotide binding
Biological process: oxidation-reduction process
Cellular component: not assigned

EC 1.5.1.33: Pteridine reductase

Reaction catalysed:
5,6,7,8-tetrahydrobiopterin + 2 NADP(+) = biopterin + 2 NADPH.
Comments:
  • The enzyme from Leishmania (both amastigote and promastigote forms) catalyzes the NADPH-dependent reduction of folate and a wide variety of unconjugated pterins, including biopterin, to their tetrahydro forms.
  • It also catalyzes the reduction of 7,8-dihydropterins and 7,8- dihydrofolate to their tetrahydro forms.
  • In contrast to EC 1.5.1.3 and EC 1.5.1.34, pteridine reductase will not catalyze the reduction of the quinonoid form of dihydrobiopterin.
  • The enzyme is specific for NADPH; no activity has been detected with NADH.
  • It also differs from EC 1.5.1.3 in being specific for the B side of NADPH.
  • Formerly EC 1.1.1.253.
Systematic name:
5,6,7,8-tetrahydrobiopterin:NADP(+) oxidoreductase
Alternative Name(s):
  • PTR1
  • Pteridine reductase 1

GO terms

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Sequence families

Pfam Protein families (Pfam)
PF00106
Domain description: short chain dehydrogenase
Occurring in:
  1. Pteridine reductase
1 copy of Pfam domain PF00106 (short chain dehydrogenase) in Pteridine reductase in PDB 2c7v.

PF13561
Domain description: Enoyl-(Acyl carrier protein) reductase
Occurring in:
  1. Pteridine reductase
1 copy of Pfam domain PF13561 (Enoyl-(Acyl carrier protein) reductase) in Pteridine reductase in PDB 2c7v.

InterPro InterPro annotations
IPR002347
Domain description: Short-chain dehydrogenase/reductase SDR
Occurring in:
  1. Pteridine reductase
IPR036291
Domain description: NAD(P)-binding domain superfamily
Occurring in:
  1. Pteridine reductase
IPR014058
Domain description: Pteridine reductase
Occurring in:
  1. Pteridine reductase
IPR020904
Domain description: Short-chain dehydrogenase/reductase, conserved site
Occurring in:
  1. Pteridine reductase

Structure domain

CATH CATH domain
3.40.50.720
Class: Alpha Beta
Architecture: 3-Layer(aba) Sandwich
Topology: Rossmann fold
Homology: NAD(P)-binding Rossmann-like Domain
Occurring in:
  1. Pteridine reductase
1 copy of CATH domain 3.40.50.720 (Rossmann fold) in Pteridine reductase in PDB 2c7v.