PDBe 2c7v

X-ray diffraction
2.2Å resolution

STRUCTURE OF TRYPANOSOMA BRUCEI PTERIDINE REDUCTASE (PTR1) IN TERNARY COMPLEX WITH COFACTOR AND THE ANTIFOLATE METHOTREXATE

Released:

Function and Biology Details

Reaction catalysed:
5,6,7,8-tetrahydrobiopterin + 2 NADP(+) = biopterin + 2 NADPH. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pteridine reductase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 268 amino acids
Theoretical weight: 28.74 KDa
Source organism: Trypanosoma brucei brucei
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O76290 (Residues: 1-268; Coverage: 100%)
Gene name: PTR1
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P21
Unit cell:
a: 74.655Å b: 90.244Å c: 82.841Å
α: 90° β: 115.76° γ: 90°
R-values:
R R work R free
0.157 0.153 0.223
Expression system: Escherichia coli BL21(DE3)