› 2c7t

X-ray diffraction
2.1Å resolution

CRYSTAL STRUCTURE OF THE PLP-BOUND FORM OF BTRR, A DUAL FUNCTIONAL AMINOTRANSFERASE INVOLVED IN BUTIROSIN BIOSYNTHESIS.

Released:
DOI: 10.2210/pdb2c7t/pdb
PDB entry 2c7t coloured by chain, front view.
PDB entry 2c7t coloured by chain, side view.
PDB entry 2c7t coloured by chain, top view.
Source organism: Niallia circulans
Primary publication:
Crystal structures of the PLP- and PMP-bound forms of BtrR, a dual functional aminotransferase involved in butirosin biosynthesis.
Popovic B, Tang X, Chirgadze DY, Huang F, Blundell TL, Spencer JB
Proteins 65 220-30 (2006)
PMID: 16894611

Function and Biology Details

Reactions catalysed: 
L-glutamine + 2-deoxy-scyllo-inosose = 2-oxoglutaramate + 2-deoxy-scyllo-inosamine
L-glutamine + 3-amino-2,3-dideoxy-scyllo-inosose = 2-oxoglutaramate + 2-deoxystreptamine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184463 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-glutamine:2-deoxy-scyllo-inosose aminotransferase Chain: A
Molecule details ›
Chain: A
Length: 418 amino acids
Theoretical weight: 47.09 KDa
Source organism: Niallia circulans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8G8Y2 (Residues: 1-418; Coverage: 100%)
Gene names: btrR, btrS
Sequence domains: DegT/DnrJ/EryC1/StrS aminotransferase family
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.5
Spacegroup: P3221
Unit cell:
a: 73.74Å b: 73.74Å c: 162.52Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.174 0.174 0.206
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics.
Llewellyn et al. (2006)
3 more

3 mentions without citation

Structure and mutagenic conversion of E1 dehydrase: at the crossroads of dehydration, amino transfer, and epimerization.
Smith et al. (2008)
2 more