› 2c7f

X-ray diffraction
2.7Å resolution

The Structure of a family 51 arabinofuranosidase, Araf51, from Clostridium thermocellum in complex with 1,5-alpha-L-Arabinotriose.

Released:
DOI: 10.2210/pdb2c7f/pdb
PDB entry 2c7f coloured by chain, front view.
PDB entry 2c7f coloured by chain, side view.
PDB entry 2c7f coloured by chain, top view.
Source organism: Acetivibrio thermocellus
Primary publication:
Structural insight into the ligand specificity of a thermostable family 51 arabinofuranosidase, Araf51, from Clostridium thermocellum.
Taylor EJ, Smith NL, Turkenburg JP, D'Souza S, Gilbert HJ, Davies GJ
Biochem J 395 31-7 (2006)
PMID: 16336192

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-107072 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Intracellular exo-alpha-(1->5)-L-arabinofuranosidase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 513 amino acids
Theoretical weight: 58.91 KDa
Source organism: Acetivibrio thermocellus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A3DIH0 (Residues: 1-503; Coverage: 100%)
Gene name: Cthe_2548
Sequence domains: Alpha-L-arabinofuranosidase C-terminal domain
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: AHR
Carbohydrate polymer
Carbohydrate polymer : NEW Components: AHR
Carbohydrate polymer
1 bound ligand:
Ligand EDO 9 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P43212
Unit cell:
a: 173.341Å b: 173.341Å c: 272.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.243 0.242 0.255
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Thermostable enzymes as biocatalysts in the biofuel industry.
Yeoman et al. (2010)
6 more

4 mentions without citation

Structural insight into the ligand specificity of a thermostable family 51 arabinofuranosidase, Araf51, from Clostridium thermocellum.
Taylor et al. (2006)
3 more