› 2c5j

X-ray diffraction
2.1Å resolution

N-terminal domain of tlg1, domain-swapped dimer

Released:
DOI: 10.2210/pdb2c5j/pdb
PDB entry 2c5j coloured by chain, front view.
PDB entry 2c5j coloured by chain, side view.
PDB entry 2c5j coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural analysis of the interaction between the SNARE Tlg1 and Vps51.
Fridmann-Sirkis Y, Kent HM, Lewis MJ, Evans PR, Pelham HR
Traffic 7 182-90 (2006)
PMID: 16420526

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169875 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
t-SNARE affecting a late Golgi compartment protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 95 amino acids
Theoretical weight: 11.24 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q03322 (Residues: 1-95; Coverage: 42%)
Gene names: D8035.11, TLG1, YDR468C
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 44.666Å b: 46.104Å c: 117.887Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.259 0.256 0.311
Expression system: Escherichia coli BL21

Quick links

Citations

11 review citations

Coats, tethers, Rabs, and SNAREs work together to mediate the intracellular destination of a transport vesicle.
Cai et al. (2007)
10 more

4 mentions without citation

Protein acrobatics in pairs--dimerization via domain swapping.
Gronenborn AM. (2009)
3 more