› 2c5b

X-ray diffraction
2.5Å resolution

X-ray crystal structure of 5'-fluorodeoxyadenosine synthase from Streptomyces cattleya complexed with 2'deoxy-5'deoxy-fluoroadenosine.

Released:
DOI: 10.2210/pdb2c5b/pdb
PDB entry 2c5b coloured by chain, front view.
PDB entry 2c5b coloured by chain, side view.
PDB entry 2c5b coloured by chain, top view.
Source organism: Streptomyces cattleya
Primary publication:
Substrate specificity in enzymatic fluorination. The fluorinase from Streptomyces cattleya accepts 2'-deoxyadenosine substrates.
Cobb SL, Deng H, McEwan AR, Naismith JH, O'Hagan D, Robinson DA
Org Biomol Chem 4 1458-60 (2006)
PMID: 16604208

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + fluoride = 5'-deoxy-5'-fluoroadenosine + L-methionine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180682 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fluorinase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 299 amino acids
Theoretical weight: 32.4 KDa
Source organism: Streptomyces cattleya
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q70GK9 (Residues: 1-299; Coverage: 100%)
Gene name: flA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MET 3 x MET
Ligand 5F1 3 x 5F1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: C2221
Unit cell:
a: 76.153Å b: 129.669Å c: 183.165Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.189 0.267
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

Fluoro amino acids: a rarity in nature, yet a prospect for protein engineering.
Odar et al. (2015)
3 more

3 mentions without citation

Halogenases: a palette of emerging opportunities for synthetic biology-synthetic chemistry and C-H functionalisation.
Crowe et al. (2021)
2 more