PDBe 2c0b

X-ray diffraction
3.18Å resolution

Catalytic domain of E. coli RNase E in complex with 13-mer RNA


Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Ribonuclease E Chain: L
Molecule details ›
Chain: L
Length: 517 amino acids
Theoretical weight: 58.22 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P21513 (Residues: 1-510; Coverage: 48%)
Gene names: JW1071, ams, b1084, hmp1, rne
Sequence domains:
5'-R(*UP*UP*UP*AP*CP*AP*GP*UP*AP*UP*UP*UP*G)-3' Chain: R
Molecule details ›
Chain: R
Length: 13 nucleotides
Theoretical weight: 4.08 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P6222
Unit cell:
a: 196.049Å b: 196.049Å c: 142.66Å
α: 90° β: 90° γ: 120°
R R work R free
0.254 0.252 0.282
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided