PDBe 2bzi

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF THE HUMAN PIM1 IN COMPLEX WITH A RUTHENIUM ORGANOMETALLIC LIGAND RU2

Released:
Source organism: Homo sapiens
Entry authors: Debreczeni JE, Bullock A, Knapp S, Von Delft F, Sundstrom M, Arrowsmith C, Weigelt J, Edwards A

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase pim-1 Chain: B
Molecule details ›
Chain: B
Length: 313 amino acids
Theoretical weight: 35.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P11309 (Residues: 92-404; Coverage: 78%)
Gene name: PIM1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P65
Unit cell:
a: 98.241Å b: 98.241Å c: 80.891Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.218 0.216 0.265
Expression system: Escherichia coli BL21