PDBe 2bz8

X-ray diffraction
2Å resolution

N-terminal Sh3 domain of CIN85 bound to Cbl-b peptide

Released:
Source organism: Homo sapiens

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)- ubiquitinyl-[acceptor protein]-L-lysine. 
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
SH3 domain-containing kinase-binding protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 58 amino acids
Theoretical weight: 6.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96B97 (Residues: 1-58; Coverage: 9%)
Gene names: CIN85, SH3KBP1
Sequence domains: Variant SH3 domain
Structure domains: SH3 Domains
E3 ubiquitin-protein ligase CBL-B Chain: C
Molecule details ›
Chain: C
Length: 11 amino acids
Theoretical weight: 1.33 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q13191 (Residues: 902-912; Coverage: 1%)
Gene names: CBLB, Nbla00127, RNF56

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER
Spacegroup: P65
Unit cell:
a: 51.158Å b: 51.158Å c: 97.472Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.221 0.221 0.271
Expression systems:
  • Escherichia coli
  • Not provided