PDBe 2bv9

X-ray diffraction
1.5Å resolution

HOW FAMILY 26 GLYCOSIDE HYDROLASES ORCHESTRATE CATALYSIS ON DIFFERENT POLYSACCHARIDES. STRUCTURE AND ACTIVITY OF A CLOSTRIDIUM THERMOCELLUM LICHENASE, CtLIC26A

Released:

Function and Biology Details

Reaction catalysed:
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans. 
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoglucanase H Chain: A
Molecule details ›
Chain: A
Length: 290 amino acids
Theoretical weight: 33.19 KDa
Source organism: Ruminiclostridium thermocellum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P16218 (Residues: 26-304; Coverage: 30%)
Gene names: Cthe_1472, celH
Sequence domains: Glycosyl hydrolase family 26
Structure domains: Glycosidases

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 49.828Å b: 63.646Å c: 100.021Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.132 0.131 0.157
Expression system: Escherichia coli BL21(DE3)