2btt Experiments and Validation

Solution NMR

Source organism: Saccharomyces cerevisiae
Refinement method: ARIA 1.2
Number of deposited models: 20 models
Chemical shifts: BMR6197  

Sample information

Author description:
NMR STRUCTURE OF MYO3-SH3 DOMAIN FROM MYOSIN-TYPE I FROM S. CEREVISIAE
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli

Validation information

 
Structure ensemble information
Refinement method: ARIA 1.2
Number of calculated models: 50
Number of deposited models: 20 (LOWEST ENERGY)
Representative model: 1 (author defined)
 
Experimental NMR data
Chemical shifts: BMR6197  

Experimental information

Sample 1
Contents: 90% WATER/10% D2O Recorded spectra: 1H-15N-NOESY-HSQC, 1H-15N- TOCSY-HSQC, HNHA, HNHB, CBCACONH, CBCANH, HNCO, HCCH- TOCSY, 2D-NOESY, 2D COSY, 2D-TOCSY

Samples and spectra
NMR spectrometers used
VARIAN UNITYINOVA 500 MHz, 600 MHz, 800 MHz