PDBe 2bsx

X-ray diffraction
2Å resolution

Crystal structure of the Plasmodium falciparum purine nucleoside phosphorylase complexed with inosine

Released:

Function and Biology Details

Reaction catalysed:
(1) Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate. (2) Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Purine nucleoside phosphorylase Chain: A
Molecule details ›
Chain: A
Length: 253 amino acids
Theoretical weight: 27.96 KDa
Source organism: Plasmodium falciparum 3D7
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8I3X4 (Residues: 1-245; Coverage: 100%)
Gene name: PF3D7_0513300
Sequence domains: Phosphorylase superfamily
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P321
Unit cell:
a: 95.117Å b: 95.117Å c: 47.075Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.268 0.264 0.342
Expression system: Escherichia coli BL21