PDBe 2bp7

X-ray diffraction
2.9Å resolution

New crystal form of the Pseudomonas putida branched-chain dehydrogenase (E1)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
2-oxoisovalerate dehydrogenase subunit beta Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 339 amino acids
Theoretical weight: 37.17 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09061 (Residues: 1-339; Coverage: 100%)
Gene name: bkdA2
Sequence domains:
Structure domains:
2-oxoisovalerate dehydrogenase subunit alpha Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 410 amino acids
Theoretical weight: 45.32 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09060 (Residues: 1-410; Coverage: 100%)
Gene name: bkdA1
Sequence domains:
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P6122
Unit cell:
a: 156.866Å b: 156.866Å c: 619.605Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.228 0.227 0.254
Expression system: Escherichia coli BL21(DE3)