PDBe 2bmi

X-ray diffraction
2Å resolution

METALLO-BETA-LACTAMASE

Released:
Source organism: Bacteroides fragilis

Function and Biology Details

Reaction catalysed:
A beta-lactam + H(2)O = a substituted beta-amino acid. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metallo-beta-lactamase type 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 232 amino acids
Theoretical weight: 25.36 KDa
Source organism: Bacteroides fragilis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P25910 (Residues: 18-249; Coverage: 100%)
Gene names: ccrA, cfiA
Structure domains: Metallo-beta-lactamase, chain A

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 47.256Å b: 94.92Å c: 111.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 0.262
Expression system: Escherichia coli BL21