PDBe 2bl0

X-ray diffraction
1.75Å resolution

Physarum polycephalum myosin II regulatory domain

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Myosin regulatory light chain Chain: B
Molecule details ›
Chain: B
Length: 145 amino acids
Theoretical weight: 15.94 KDa
Source organism: Physarum polycephalum
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P08053 (Residues: 1-145; Coverage: 99%)
Sequence domains:
Structure domains: EF-hand
Myosin regulatory light chain Chain: C
Molecule details ›
Chain: C
Length: 142 amino acids
Theoretical weight: 16.2 KDa
Source organism: Physarum polycephalum
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8WSQ4 (Residues: 14-155; Coverage: 90%)
Gene name: RMLC
Sequence domains: EF-hand domain pair
Structure domains: EF-hand
Major plasmodial myosin heavy chain Chain: A
Molecule details ›
Chain: A
Length: 63 amino acids
Theoretical weight: 7.58 KDa
Source organism: Physarum polycephalum
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9BJD3 (Residues: 778-840; Coverage: 3%)
Gene name: mynP1

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P212121
Unit cell:
a: 55.574Å b: 70.858Å c: 97.877Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.219 0.252
Expression system: Escherichia coli BL21