PDBe 2bkk

X-ray diffraction
2.15Å resolution

Crystal structure of Aminoglycoside Phosphotransferase APH(3')-IIIa in complex with the inhibitor AR_3a

Released:

Function and Biology Details

Reaction catalysed:
ATP + kanamycin = ADP + kanamycin 3'-phosphate. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Aminoglycoside 3'-phosphotransferase Chains: A, C
Molecule details ›
Chains: A, C
Length: 264 amino acids
Theoretical weight: 30.98 KDa
Source organism: Enterococcus faecalis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A3Y5 (Residues: 1-264; Coverage: 100%)
Gene name: aphA
Sequence domains: Phosphotransferase enzyme family
Structure domains:
DESIGNED ANKYRIN REPEAT INHIBITOR AR_3A Chains: B, D
Molecule details ›
Chains: B, D
Length: 169 amino acids
Theoretical weight: 18.6 KDa
Source organism: synthetic construct
Expression system: Escherichia coli
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 59.67Å b: 98.082Å c: 81.3Å
α: 90° β: 110.01° γ: 90°
R-values:
R R work R free
0.2 0.199 0.26
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli