PDBe 2bfx

X-ray diffraction
1.8Å resolution

Mechanism of Aurora-B activation by INCENP and inhibition by Hesperadin.

Released:

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein. 
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Aurora kinase B-A Chains: A, B
Molecule details ›
Chains: A, B
Length: 284 amino acids
Theoretical weight: 33.54 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6DE08 (Residues: 78-361; Coverage: 79%)
Gene names: airk2-a, aurkb-a
Sequence domains: Protein kinase domain
Structure domains:
Inner centromere protein A Chains: C, D
Molecule details ›
Chains: C, D
Length: 43 amino acids
Theoretical weight: 4.98 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O13024 (Residues: 798-840; Coverage: 5%)
Gene name: incenp-a

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21
Unit cell:
a: 45.94Å b: 66.9Å c: 116.52Å
α: 90° β: 96.79° γ: 90°
R-values:
R R work R free
0.197 0.197 0.226
Expression system: Escherichia coli BL21(DE3)