PDBe 2bcj

X-ray diffraction
3.06Å resolution

Crystal Structure of G Protein-Coupled Receptor Kinase 2 in Complex with Galpha-q and Gbetagamma Subunits

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Beta-adrenergic receptor kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 689 amino acids
Theoretical weight: 79.75 KDa
Source organism: Bos taurus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P21146 (Residues: 1-689; Coverage: 100%)
Gene names: ADRBK1, GRK2
Sequence domains:
Structure domains:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 340 amino acids
Theoretical weight: 37.31 KDa
Source organism: Bos taurus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P62871 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 71 amino acids
Theoretical weight: 7.85 KDa
Source organism: Bos taurus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: P63212 (Residues: 1-71; Coverage: 100%)
Gene name: GNG2
Sequence domains: GGL domain
Structure domains: Transducin (heterotrimeric G protein), gamma chain
Guanine nucleotide-binding protein G(q) subunit alpha; Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: Q
Molecule details ›
Chain: Q
Length: 353 amino acids
Theoretical weight: 41.3 KDa
Source organisms: Expression system: Trichoplusia ni
UniProt:
  • Canonical: P10824 (Residues: 2-28; Coverage: 8%)
  • Canonical: P21279 (Residues: 7-7, 37-359; Coverage: 90%)
  • nullnull
Gene names: Gnai-1, Gnai1, Gnaq
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21
Unit cell:
a: 64.918Å b: 129.954Å c: 122.764Å
α: 90° β: 95.81° γ: 90°
R-values:
R R work R free
0.236 0.243 0.292
Expression system: Trichoplusia ni