› 2b2n

X-ray diffraction
2.1Å resolution

Structure of transcription-repair coupling factor

Released:
DOI: 10.2210/pdb2b2n/pdb
PDB entry 2b2n coloured by chain, front view.
PDB entry 2b2n coloured by chain, side view.
PDB entry 2b2n coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural basis for transcription-coupled repair: the N terminus of Mfd resembles UvrB with degenerate ATPase motifs.
Assenmacher N, Wenig K, Lammens A, Hopfner KP
J Mol Biol 355 675-83 (2006)
PMID: 16309703

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151850 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription-repair-coupling factor Chains: A, B
Molecule details ›
Chains: A, B
Length: 344 amino acids
Theoretical weight: 39.4 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P30958 (Residues: 1-333; Coverage: 29%)
Gene names: JW1100, b1114, mfd
Sequence domains: UvrB interaction domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand NA 3 x NA
Ligand P4C 2 x P4C
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P6522
Unit cell:
a: 112.65Å b: 112.65Å c: 213.86Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.198 0.198 0.232
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Repair of UV damage in bacteria.
Goosen et al. (2008)
8 more

1 mention without citation

Molecular determinants for dsDNA translocation by the transcription-repair coupling and evolvability factor Mfd.
Brugger et al. (2020)