PDBe 2b0q

X-ray diffraction
2.7Å resolution

Crystal Structure Of 3',5"-Aminoglycoside Phosphotransferase Type IIIa ADP Neomycin B Complex


Function and Biology Details

Reaction catalysed:
ATP + kanamycin = ADP + kanamycin 3'-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Aminoglycoside 3'-phosphotransferase Chain: A
Molecule details ›
Chain: A
Length: 263 amino acids
Theoretical weight: 30.88 KDa
Source organism: Enterococcus faecalis
Expression system: Escherichia coli BL21
  • Canonical: P0A3Y5 (Residues: 2-264; Coverage: 100%)
Gene name: aphA
Sequence domains: Phosphotransferase enzyme family
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P4322
Unit cell:
a: 46.357Å b: 46.357Å c: 301.613Å
α: 90° β: 90° γ: 90°
R R work R free
0.225 0.225 0.312
Expression system: Escherichia coli BL21