PDBe 2b08

X-ray diffraction
1.9Å resolution

Reduced acetamide-bound M150G Nitrite Reductase from Alcaligenes faecalis

Released:

Function and Biology Details

Reaction catalysed:
Nitric oxide + H(2)O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H(+). 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Copper-containing nitrite reductase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 340 amino acids
Theoretical weight: 36.73 KDa
Source organism: Alcaligenes faecalis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P38501 (Residues: 37-376; Coverage: 99%)
Gene names: nir, nirK
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 60.972Å b: 102.047Å c: 146.423Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.177 0.22
Expression system: Escherichia coli BL21(DE3)