› 2azo

X-ray diffraction
2.3Å resolution

DNA MISMATCH REPAIR PROTEIN MUTH FROM E. COLI

Released:
DOI: 10.2210/pdb2azo/pdb
PDB entry 2azo coloured by chain, front view.
PDB entry 2azo coloured by chain, side view.
PDB entry 2azo coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural basis for MutH activation in E.coli mismatch repair and relationship of MutH to restriction endonucleases.
Ban C, Yang W
EMBO J 17 1526-34 (1998)
PMID: 9482749

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139160 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA mismatch repair protein MutH Chains: A, B
Molecule details ›
Chains: A, B
Length: 232 amino acids
Theoretical weight: 25.84 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P06722 (Residues: 1-229; Coverage: 100%)
Gene names: JW2799, b2831, mutH, mutR, prv
Sequence domains: DNA mismatch repair enzyme MutH
Structure domains: DNA mismatch repair MutH/Restriction endonuclease, type II

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 39.86Å b: 111.57Å c: 54.93Å
α: 90° β: 96.53° γ: 90°
R-values:
R R work R free
0.202 0.202 0.293
Expression system: Escherichia coli

Quick links

Citations

25 review citations

DNA mismatch repair.
Kunkel et al. (2005)
24 more

12 mentions without citation

Evolution of the methyl directed mismatch repair system in Escherichia coli.
Putnam CD. (2016)
11 more