PDBe 2az5

X-ray diffraction
2.1Å resolution

Crystal Structure of TNF-alpha with a small molecule inhibitor

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tumor necrosis factor, soluble form Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 148 amino acids
Theoretical weight: 16.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01375 (Residues: 86-233; Coverage: 64%)
Gene names: TNF, TNFA, TNFSF2
Sequence domains: TNF(Tumour Necrosis Factor) family
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: R3
Unit cell:
a: 165.254Å b: 165.254Å c: 63.728Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.227 0.22 0.278
Expression system: Escherichia coli BL21(DE3)