PDBe 2ayh

X-ray diffraction
1.6Å resolution

CRYSTAL AND MOLECULAR STRUCTURE AT 1.6 ANGSTROMS RESOLUTION OF THE HYBRID BACILLUS ENDO-1,3-1,4-BETA-D-GLUCAN 4-GLUCANOHYDROLASE H(A16-M)

Released:

Function and Biology Details

Reaction catalysed:
Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds. 
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-glucanase Chain: A
Molecule details ›
Chain: A
Length: 214 amino acids
Theoretical weight: 23.94 KDa
Source organism: hybrid
Expression system: Escherichia coli
UniProt:
  • Canonical: P23904 (Residues: 25-237; Coverage: 100%)
Sequence domains: Glycosyl hydrolases family 16
Structure domains: Jelly Rolls

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 64.18Å b: 78.26Å c: 39.23Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.143 not available not available
Expression system: Escherichia coli