PDBe 2at3

X-ray diffraction
1Å resolution

1.00 A Crystal Structure Of L123V/L133V Mutant of Nitrophorin 4 From Rhodnius Prolixus Complexed With Imidazole at pH 5.6

Released:
Source organism: Rhodnius prolixus
Entry authors: Amoia AM, Montfort WR

Function and Biology Details

Reaction catalysed:
3 nitrite + 2 H(+) = 2 nitric oxide + nitrate + H(2)O. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrophorin-4 Chain: X
Molecule details ›
Chain: X
Length: 184 amino acids
Theoretical weight: 20.26 KDa
Source organism: Rhodnius prolixus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q94734 (Residues: 22-205; Coverage: 100%)
Sequence domains: Nitrophorin
Structure domains: Lipocalin

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: C2
Unit cell:
a: 70.221Å b: 42.743Å c: 52.886Å
α: 90° β: 94.09° γ: 90°
R-values:
R R work R free
0.145 0.144 0.167
Expression system: Escherichia coli BL21(DE3)