PDBe 2ast

X-ray diffraction
2.3Å resolution

Crystal structure of Skp1-Skp2-Cks1 in complex with a p27 peptide

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
hetero octamer
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
S-phase kinase-associated protein 2 Chain: B
Molecule details ›
Chain: B
Length: 336 amino acids
Theoretical weight: 37.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13309 (Residues: 89-424; Coverage: 79%)
Gene names: FBXL1, SKP2
Sequence domains: F-box-like
Structure domains: Ribonuclease Inhibitor
Cyclin-dependent kinase inhibitor 1B Chain: D
Molecule details ›
Chain: D
Length: 10 amino acids
Theoretical weight: 1.13 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P46527 (Residues: 181-190; Coverage: 5%)
Gene names: CDKN1B, KIP1
S-phase kinase-associated protein 1 Chain: A
Molecule details ›
Chain: A
Length: 159 amino acids
Theoretical weight: 18.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63208 (Residues: 3-160; Coverage: 97%)
Gene names: EMC19, OCP2, SKP1, SKP1A, TCEB1L
Sequence domains: Skp1 family, dimerisation domain
Structure domains: Potassium Channel Kv1.1; Chain A
Cyclin-dependent kinases regulatory subunit 1 Chain: C
Molecule details ›
Chain: C
Length: 69 amino acids
Theoretical weight: 8.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61024 (Residues: 5-73; Coverage: 87%)
Gene names: CKS1, CKS1B, PNAS-143, PNAS-16
Sequence domains: Cyclin-dependent kinase regulatory subunit
Structure domains: Cell cycle regulatory proteins

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P3221
Unit cell:
a: 148.7Å b: 148.7Å c: 98.8Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.202 0.221
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided