PDBe 2ass

X-ray diffraction
3Å resolution

Crystal structure of the Skp1-Skp2-Cks1 complex

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero hexamer
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
S-phase kinase-associated protein 2 Chain: B
Molecule details ›
Chain: B
Length: 336 amino acids
Theoretical weight: 37.92 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q13309 (Residues: 89-424; Coverage: 79%)
Gene names: FBXL1, SKP2
Sequence domains: F-box-like
Structure domains: Ribonuclease Inhibitor
S-phase kinase-associated protein 1 Chain: A
Molecule details ›
Chain: A
Length: 159 amino acids
Theoretical weight: 18.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63208 (Residues: 3-160; Coverage: 97%)
Gene names: EMC19, OCP2, SKP1, SKP1A, TCEB1L
Sequence domains: Skp1 family, dimerisation domain
Structure domains: Potassium Channel Kv1.1; Chain A
Cyclin-dependent kinases regulatory subunit 1 Chain: C
Molecule details ›
Chain: C
Length: 69 amino acids
Theoretical weight: 8.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61024 (Residues: 5-73; Coverage: 87%)
Gene names: CKS1, CKS1B, PNAS-143, PNAS-16
Sequence domains: Cyclin-dependent kinase regulatory subunit
Structure domains: Cell cycle regulatory proteins

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9A
Spacegroup: P3221
Unit cell:
a: 149.332Å b: 149.332Å c: 99.916Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 0.214 0.242
Expression system: Escherichia coli BL21(DE3)