2aoq

X-ray diffraction
2.2Å resolution

Crystal structure of MutH-unmethylated DNA complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-115078 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA mismatch repair protein MutH Chain: A
Molecule details ›
Chain: A
Length: 223 amino acids
Theoretical weight: 24.94 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P44688 (Residues: 1-223; Coverage: 100%)
Gene names: HI_0403, mutH
Sequence domains: DNA mismatch repair enzyme MutH
Structure domains: DNA mismatch repair MutH/Restriction endonuclease, type II
5'-D(*GP*CP*AP*TP*GP*AP*TP*CP*AP*TP*GP*C)-3' Chains: B, C
Molecule details ›
Chains: B, C
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: Haemophilus influenzae
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P63
Unit cell:
a: 114.624Å b: 114.624Å c: 46.755Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.199 0.22
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided