PDBe 2akj

X-ray diffraction
2.8Å resolution

Structure of spinach nitrite reductase

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferredoxin--nitrite reductase, chloroplastic Chain: A
Molecule details ›
Chain: A
Length: 608 amino acids
Theoretical weight: 68.02 KDa
Source organism: Spinacia oleracea
Expression system: Escherichia coli
UniProt:
  • Canonical: P05314 (Residues: 33-594; Coverage: 95%)
Gene name: NIR
Sequence domains:
Structure domains: Sulfite Reductase Hemoprotein, domain 1

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200HB
Spacegroup: P41212
Unit cell:
a: 128.97Å b: 128.97Å c: 120.95Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.252 0.25 0.3
Expression system: Escherichia coli