PDBe 2ak5

X-ray diffraction
1.85Å resolution

beta PIX-SH3 complexed with a Cbl-b peptide


Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)- ubiquitinyl-[acceptor protein]-L-lysine. 
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Rho guanine nucleotide exchange factor 7 Chains: A, B
Molecule details ›
Chains: A, B
Length: 64 amino acids
Theoretical weight: 7.29 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
  • Canonical: O55043 (Residues: 5-66; Coverage: 10%)
Gene names: Arhgef7, Pak3bp, Pixb
Sequence domains: Variant SH3 domain
Structure domains: SH3 Domains
E3 ubiquitin-protein ligase CBL-B Chain: D
Molecule details ›
Chain: D
Length: 8 amino acids
Theoretical weight: 1.01 KDa
Source organism: Homo sapiens
Expression system: Not provided
  • Canonical: Q13191 (Residues: 904-911; Coverage: 1%)
Gene names: CBLB, Nbla00127, RNF56

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX14.2
Spacegroup: P3221
Unit cell:
a: 69.338Å b: 69.338Å c: 58.113Å
α: 90° β: 90° γ: 120°
R R work R free
0.22 0.227 0.269
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided