PDBe 2ak4

X-ray diffraction
2.5Å resolution

Crystal Structure of SB27 TCR in complex with HLA-B*3508-13mer peptide

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, B-35 alpha chain Chains: A, F, K, Q
Molecule details ›
Chains: A, F, K, Q
Length: 276 amino acids
Theoretical weight: 31.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P30685 (Residues: 25-300; Coverage: 82%)
Gene names: HLA-B, HLAB
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, G, L, R
Molecule details ›
Chains: B, G, L, R
Length: 99 amino acids
Theoretical weight: 11.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Trans-activator protein BZLF1 Chains: C, H, M, S
Molecule details ›
Chains: C, H, M, S
Length: 13 amino acids
Theoretical weight: 1.43 KDa
Source organism: Human herpesvirus 4 strain B95-8
Expression system: Not provided
UniProt:
  • Canonical: P03206 (Residues: 52-64; Coverage: 5%)
Gene name: BZLF1
T-cell receptor beta-1 chain C region Chains: E, J, P, U
Molecule details ›
Chains: E, J, P, U
Length: 245 amino acids
Theoretical weight: 27.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01850 (Residues: 1-130; Coverage: 73%)
Gene name: TRBC1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
T-cell receptor alpha chain C region Chains: D, I, N, T
Molecule details ›
Chains: D, I, N, T
Length: 211 amino acids
Theoretical weight: 24.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01848 (Residues: 1-95; Coverage: 67%)
Gene names: TCRA, TRAC
Sequence domains: Domain of unknown function (DUF1968)
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P21
Unit cell:
a: 79.154Å b: 213.282Å c: 122.303Å
α: 90° β: 89.94° γ: 90°
R-values:
R R work R free
0.247 0.246 0.278
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided