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Novel non-heme iron center of nitrile hydratase with a claw setting of oxygen atoms.

Nagashima S, Nakasako M, Dohmae N, Tsujimura M, Takio K, Odaka M, Yohda M, Kamiya N, Endo I
Nat Struct Biol 5 347-51 (1998)
Cited: 125 times
EuropePMC logo PMID: 9586994

Abstract

The iron-containing nitrile hydratase (NHase) is a photoreactive enzyme that is inactivated in the dark because of persistent association with NO and activated by photo-dissociation of NO. The crystal structure at 1.7 A resolution and mass spectrometry revealed the structure of the non-heme iron catalytic center in the nitrosylated state. Two Cys residues coordinated to the iron were post-translationally modified to Cys-sulfenic and -sulfinic acids. Together with another oxygen atom of the Ser ligand, these modifications induced a claw setting of oxygen atoms capturing an NO molecule. This unprecedented structure is likely to enable the photo-regulation of NHase and will provide an excellent model for designing photo-controllable chelate complexes and, ultimately, proteins.

Articles - 2ahj mentioned but not cited (7)

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Related citations provided by authors (1)

  1. Crystallization of a Photosensitive Nitrile Hydratase from Rhodococcus Sp. N-771. Nagamune T, Honda J, Cho WD, Kamiya N, Teratani Y, Hirata A, Sasabe H, Endo I J. Mol. Biol. 220 221- (1991)

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