PDBe 2ahj

X-ray diffraction
1.7Å resolution

NITRILE HYDRATASE COMPLEXED WITH NITRIC OXIDE

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
hetero octamer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nitrile hydratase subunit alpha Chains: A, C
Molecule details ›
Chains: A, C
Length: 206 amino acids
Theoretical weight: 22.93 KDa
Source organism: Rhodococcus erythropolis
UniProt:
  • Canonical: P13448 (Residues: 2-207; Coverage: 100%)
Gene name: nthA
Sequence domains: Nitrile hydratase, alpha chain
Structure domains: Nitrile Hydratase; Chain A
Nitrile hydratase subunit beta Chains: B, D
Molecule details ›
Chains: B, D
Length: 212 amino acids
Theoretical weight: 23.51 KDa
Source organism: Rhodococcus erythropolis
UniProt:
  • Canonical: P13449 (Residues: 1-212; Coverage: 100%)
Gene names: nha2, nthB
Sequence domains: Nitrile hydratase beta subunit
Structure domains:

Ligands and Environments


2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-18B
Spacegroup: P21212
Unit cell:
a: 117.4Å b: 145.6Å c: 52.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.179 0.228