› 2aey

X-ray diffraction
3.27Å resolution

Crystal structure of fructan 1-exohydrolase IIa from Cichorium intybus in complex with 2,5 dideoxy-2,5-immino-D-mannitol

Released:
DOI: 10.2210/pdb2aey/pdb
PDB entry 2aey coloured by chain, front view.
PDB entry 2aey coloured by chain, side view.
PDB entry 2aey coloured by chain, top view.
Source organism: Cichorium intybus
Primary publication:
Insights into the fine architecture of the active site of chicory fructan 1-exohydrolase: 1-kestose as substrate vs sucrose as inhibitor.
Verhaest M, Lammens W, Le Roy K, De Ranter CJ, Van Laere A, Rabijns A, Van den Ende W
New Phytol 174 90-100 (2007)
PMID: 17335500

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing (2->1)- and (2->6)-linked beta-D-fructofuranose residues in fructans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188156 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Fructan 1-exohydrolase IIa Chain: A
Molecule details ›
Chain: A
Length: 543 amino acids
Theoretical weight: 61.12 KDa
Source organism: Cichorium intybus
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q93X60 (Residues: 39-581; Coverage: 98%)
Gene name: 1-feh IIa
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand DQQ 1 x DQQ
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P41212
Unit cell:
a: 138.86Å b: 138.86Å c: 182.44Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.217 0.257
Expression system: Komagataella pastoris

Quick links

Citations

8 review citations

Sugars and plant innate immunity.
Bolouri Moghaddam et al. (2012)
7 more

1 mention without citation

An acceptor-substrate binding site determining glycosyl transfer emerges from mutant analysis of a plant vacuolar invertase and a fructosyltransferase.
Altenbach et al. (2009)