2adm Citations

Differential binding of S-adenosylmethionine S-adenosylhomocysteine and Sinefungin to the adenine-specific DNA methyltransferase M.TaqI.

J. Mol. Biol. 265 56-67 (1997)
Related entries: 1aqi, 1aqj

Cited: 62 times
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The crystal structures of the binary complexes of the DNA methyltransferase M.TaqI with the inhibitor Sinefungin and the reaction product S-adenosyl-L-homocysteine were determined, both at 2.6 A resolution. Structural comparison of these binary complexes with the complex formed by M.TaqI and the cofactor S-adenosyl-L-methionine suggests that the key element for molecular recognition of these ligands is the binding of their adenosine part in a pocket, and discrimination between cofactor, reaction product and inhibitor is mediated by different conformations of these molecules; the methionine part of S-adenosyl-L-methionine is located in the binding cleft, whereas the amino acid moieties of Sinefungin and S-adenosyl-L-homocysteine are in a different orientation and interact with the active site amino acid residues 105NPPY108. Dissociation constants for the complexes of M.TaqI with the three ligands were determined spectrofluorometrically. Sinefungin binds more strongly than S-adenosyl-L-homocysteine or S-adenosyl-L-methionine, with KD=0.34 microM, 2.4 microM and 2.0 microM, respectively.

Reviews - 2adm mentioned but not cited (1)

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Articles - 2adm mentioned but not cited (8)

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Reviews citing this publication (6)

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  20. Kinetics of methylation and binding of DNA by the EcoRV adenine-N6 methyltransferase. Jeltsch A, Friedrich T, Roth M. J. Mol. Biol. 275 747-758 (1998)
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  23. Vesicular stomatitis viruses resistant to the methylase inhibitor sinefungin upregulate RNA synthesis and reveal mutations that affect mRNA cap methylation. Li J, Chorba JS, Whelan SP. J. Virol. 81 4104-4115 (2007)
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  25. RNA:(guanine-N2) methyltransferases RsmC/RsmD and their homologs revisited--bioinformatic analysis and prediction of the active site based on the uncharacterized Mj0882 protein structure. Bujnicki JM, Rychlewski L. BMC Bioinformatics 3 10 (2002)
  26. Insights into the hyperthermostability and unusual region-specificity of archaeal Pyrococcus abyssi tRNA m1A57/58 methyltransferase. Guelorget A, Roovers M, Guérineau V, Barbey C, Li X, Golinelli-Pimpaneau B. Nucleic Acids Res. 38 6206-6218 (2010)
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  30. Crystal structures of the tRNA:m2G6 methyltransferase Trm14/TrmN from two domains of life. Fislage M, Roovers M, Tuszynska I, Bujnicki JM, Droogmans L, Versées W. Nucleic Acids Res. 40 5149-5161 (2012)
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  40. Sinefungin resistance of Saccharomyces cerevisiae arising from Sam3 mutations that inactivate the AdoMet transporter or from increased expression of AdoMet synthase plus mRNA cap guanine-N7 methyltransferase. Zheng S, Shuman S, Schwer B. Nucleic Acids Res. 35 6895-6903 (2007)
  41. Structural and functional analyses of the archaeal tRNA m2G/m22G10 methyltransferase aTrm11 provide mechanistic insights into site specificity of a tRNA methyltransferase that contains common RNA-binding modules. Hirata A, Nishiyama S, Tamura T, Yamauchi A, Hori H. Nucleic Acids Res. 44 6377-6390 (2016)
  42. 1H and 13C NMR study of the complex formed by copper(II) with the nucleoside antibiotic sinefungin. Cappannelli M, Gaggelli E, Jezowska-Bojczuk M, Molteni E, Mucha A, Porciatti E, Valensin D, Valensin G. J. Inorg. Biochem. 101 1005-1012 (2007)
  43. Structure of Type IIL Restriction-Modification Enzyme MmeI in Complex with DNA Has Implications for Engineering New Specificities. Callahan SJ, Luyten YA, Gupta YK, Wilson GG, Roberts RJ, Morgan RD, Aggarwal AK. PLoS Biol. 14 e1002442 (2016)
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  46. Crystallization and initial X-ray diffraction analysis of the tellurite-resistance S-adenosyl-L-methionine transferase protein TehB from Escherichia coli. Choudhury HG, Beis K. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 66 1496-1499 (2010)
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