PDBe 2a5d

X-ray diffraction
1.8Å resolution

Structural basis for the activation of cholera toxin by human ARF6-GTP

Released:

Function and Biology Details

Reaction catalysed:
NAD(+) + diphthamide-[translation elongation factor 2] = nicotinamide + N-(ADP-D-ribosyl)diphthamide-[translation elongation factor 2]. 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ADP-ribosylation factor 6 Chain: A
Molecule details ›
Chain: A
Length: 175 amino acids
Theoretical weight: 20.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P62330 (Residues: 1-175; Coverage: 100%)
Gene name: ARF6
Sequence domains: ADP-ribosylation factor family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Cholera enterotoxin subunit A1 Chain: B
Molecule details ›
Chain: B
Length: 193 amino acids
Theoretical weight: 21.71 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01555 (Residues: 19-210; Coverage: 80%)
Gene names: VC_1457, ctxA, toxA
Structure domains: Heat-Labile Enterotoxin, subunit A

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P212121
Unit cell:
a: 44.915Å b: 91.446Å c: 98.547Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.167 0.199
Expression system: Escherichia coli BL21(DE3)